產(chǎn)品中心

小鼠白介素17(IL17)酶聯(lián)免疫吸附檢測試劑盒

ELK1147

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse IL17(Interleukin 17) ELISA Kit
Reactivity:	Mouse
Alternative Names:	IL-17;IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
Assay Type:	Sandwich
Sensitivity:	3.2 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL17 in the samples is then determined by comparing the OD of the samples to the standard curve.

BIOSCIENCE REPORTS

Protective effects of oxymatrine against DSS-induced acute intestinal inflammation in mice via blocking the RhoA/ROCK signaling pathway

日期：2019-07-19 IF：3.84
International Journal of Ophthalmology

Ragweed pollen induces allergic conjunctivitis immune tolerance in mice via regulation of the NF-κB signal pathway

日期：2021-07-18 IF：1.779
Scientific Reports

Tofacitinib and metformin reduce the dermal thickness and fibrosis in mouse model of systemic sclerosis

日期：2022-02-15 IF：4.38
International Immunopharmacology

IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus

日期：2022-05-31 IF：5.714
iScience

Behenic acid alleviates inflammation and insulin resistance in gestational diabetes mellitus by regulating TLR4/NF-κB signaling pathway

日期：2024-10-18 IF：4.6

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	2.204	2.120
250.00	1.700	1.616
125.00	1.146	1.062
62.50	0.752	0.668
31.25	0.593	0.509
15.63	0.302	0.218
7.82	0.172	0.088
0.00	0.084	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL17 and the recovery rates were calculated by comparing the measured value to the expected amount of IL17 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	97-105%	101%
Heparin plasma(n=5)	83-99%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.