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人補(bǔ)體成分3a(C3a)酶聯(lián)免疫吸附檢測試劑盒

ELK1590

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human C3a(Complement Component 3a) ELISA Kit
Reactivity:	Human
Alternative Names:	Complement Component 3a
Assay Type:	Sandwich
Sensitivity:	30 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Immune molecule;Autoimmunity;
Uniprot ID:	P01024
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3a in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.126	2.058
2500.00	1.563	1.495
1250.00	1.186	1.118
625.00	0.872	0.804
312.50	0.509	0.441
156.25	0.325	0.257
78.13	0.225	0.157
0.00	0.068	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant C3a and the recovery rates were calculated by comparing the measured value to the expected amount of C3a in samples.

Matrix	Recovery range	Average
serum(n=5)	98-104%	102%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	96-104%	100%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.