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人低氧誘導(dǎo)因子1α(HIF1a)酶聯(lián)免疫吸附檢測試劑盒

ELK1603

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
Reactivity:	Human
Alternative Names:	HIF1-Alpha; HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Cardiovascular biology;Reproductive science;
Uniprot ID:	Q16665
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIF1a in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Clinical Medicine

Expression of Iron Metabolism Proteins in Patients with Chronic Heart Failure

日期：2022-02-22 IF：4.242
International Journal of Chronic Obstructive Pulmonary Disease

Correlation Between HIF1-A Expression and Airway Remodeling in COPD

日期：2024-04-12 IF：2.8

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.096	2.011
5.00	1.703	1.618
2.50	1.157	1.072
1.25	0.826	0.741
0.63	0.539	0.454
0.32	0.363	0.278
0.16	0.258	0.173
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HIF1a and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1a in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	87-97%	90%
Heparin plasma(n=5)	81-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.