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小鼠蛋白激酶Cε(PKCe)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1648

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Mouse PKCe(Protein Kinase C Epsilon) ELISA Kit
Reactivity:	Mouse
Alternative Names:	PKC-E; PRKCE; PRKC-E; NPKC-Epsilon
Assay Type:	Sandwich
Sensitivity:	0.61 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;
Uniprot ID:	P16054
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PKCe. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PKCe. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PKCe, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PKCe in the samples is then determined by comparing the OD of the samples to the standard curve.

The FASEB Journal

The binding of PKCε and MEG2 to STAT3 regulates IL-6-mediated microglial hyperalgesia during inflammatory pain

日期：2024-04-24 IF：4.8

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	1.842	1.747
50.00	1.647	1.552
25.00	1.035	0.940
12.50	0.752	0.657
6.25	0.541	0.446
3.13	0.336	0.241
1.57	0.213	0.118
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PKCe and the recovery rates were calculated by comparing the measured value to the expected amount of PKCe in samples.

Matrix	Recovery range	Average
serum(n=5)	87-95%	91%
EDTA plasma(n=5)	78-93%	85%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCe and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.