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人鳶尾素(Irisin)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK6625

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human Irisin ELISA Kit
Reactivity:	Human
Alternative Names:	FRCP2; Irisin; Fibronectin type III repeat-containing protein 2; FNDC5; Fibronectin Type III Domain Containing Protein 5
Assay Type:	Sandwich
Sensitivity:	6.9 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	CD & Adhesion molecule;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Irisin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Irisin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Irisin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Irisin in the samples is then determined by comparing the OD of the samples to the standard curve.

Egyptian Journal of Basic and Applied Sciences

The role of leptin, resistin, and irisin levels as prognostic indicators of lung cancer in Iraqi patients

日期：2024-06-03 IF：1.3
Diabetology & Metabolic Syndrome

Evaluation of serum irisin level and severity of erectile dysfunction in diabetic males: a cross sectional prospective study

日期：2024-09-19 IF：3.4
Molecular and Cellular Biochemistry

Resistance physical exercise modulates metabolic adipokines, decreases body weight, and improves glomerular filtration in patients with chronic kidney disease in hemodialysis

日期：2024-10-11 IF：3.5

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.185	2.092
500.00	1.633	1.540
250.00	1.253	1.160
125.00	0.872	0.779
62.50	0.559	0.466
31.25	0.329	0.236
15.63	0.225	0.132
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Irisin and the recovery rates were calculated by comparing the measured value to the expected amount of Irisin in samples.

Matrix	Recovery range	Average
serum(n=5)	83-99%	95%
EDTA plasma(n=5)	80-96%	88%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Irisin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.