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大鼠酮戊二酸脫氫酶(OGDH)酶聯(lián)免疫吸附檢測試劑盒

ELK7075

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Rat OGDH(Oxoglutarate Dehydrogenase) ELISA Kit
Reactivity:	Rat
Alternative Names:	AKGDH; E1k; OGDC; Alpha-Ketoglutarate Dehydrogenase,Lipoamide; 2-Oxoglutarate Dehydrogenase E1 Component,Mitochondrial
Assay Type:	Sandwich
Sensitivity:	1.14 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;
Uniprot ID:	Q5XI78
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OGDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OGDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OGDH in the samples is then determined by comparing the OD of the samples to the standard curve.

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標準曲線

Concentration (ng/mL)	OD	Corrected OD
200.00	2.131	2.045
100.00	1.784	1.698
50.00	1.135	1.049
25.00	0.833	0.747
12.50	0.517	0.431
6.25	0.356	0.270
3.13	0.100	0.014
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OGDH and the recovery rates were calculated by comparing the measured value to the expected amount of OGDH in samples.

Matrix	Recovery range	Average
serum(n=5)	83-98%	90%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	86-99%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OGDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.