ES1078

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

下載說明 ①

Product name:	DDDDK-Tag(binds to flag sequnence) rabbit pAb
Reactivity:	Species independent
Alternative Names:	DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
Source:	Rabbit
Dilutions:	Western Blot: 1/1000 - 1/3000. ELISA: 1:5000-20000. Not yet tested in other applications.
Immunogen:	DDDDK synthetic peptide conjugated to KLH.
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Background:	The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.