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人γ-氨基丁酸(gABA)酶聯(lián)免疫吸附檢測試劑盒

ELK0753

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Human gABA(Gamma-Aminobutyric Acid) ELISA Kit
Reactivity:	Human
Alternative Names:	4-Aminobutyric Acid
Assay Type:	Competitive Inhibition
Sensitivity:	14.6 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2.5h
Research Area:	Signal transduction;Cardiovascular biology;Neuro science;Bone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human gABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human gABA in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Hainan Medical University

Dose-response relationship between serum gamma-aminobutyric acid, oxidative stress and dyslipidemia.

日期：2024-09-30 IF：0

標準曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	0.174
1000.00	0.477
500.00	0.673
250.00	0.949
125.00	1.142
62.50	1.545
31.25	1.835
0.00	2.115

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant gABA and the recovery rates were calculated by comparing the measured value to the expected amount of gABA in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	92-107%	99%
Heparin plasma(n=5)	85-99%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of gABA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.