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人干擾素β(IFNb)酶聯(lián)免疫吸附檢測試劑盒

ELK1101

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Human IFNb(Interferon Beta) ELISA Kit
Reactivity:	Human
Alternative Names:	IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast; IFN Beta
Assay Type:	Sandwich
Sensitivity:	2.8 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P01574
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNb in the samples is then determined by comparing the OD of the samples to the standard curve.

International Immunopharmacology

Targeting the mechanism of IRF3 in sepsis-associated acute kidney injury via the Hippo pathway

日期：2023-07-06 IF：5.6
Microbial Pathogenesis

miR-342-5p targets CTNNBIP1 to promote enterovirus 71 replication

日期：2023-07-17 IF：3.8

標準曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	1.983	1.898
250.00	1.615	1.530
125.00	1.052	0.967
62.50	0.697	0.612
31.25	0.541	0.456
15.63	0.329	0.244
7.82	0.208	0.123
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IFNb and the recovery rates were calculated by comparing the measured value to the expected amount of IFNb in samples.

Matrix	Recovery range	Average
serum(n=5)	83-97%	90%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	85-95%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.