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人白介素10(IL10)酶聯(lián)免疫吸附檢測試劑盒

ELK1142

規格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻

Product name:	Human IL10(Interleukin 10) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-10;CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
Assay Type:	Sandwich
Sensitivity:	3 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Uniprot ID:	P22301
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL10 in the samples is then determined by comparing the OD of the samples to the standard curve.

Clinica Chimica Acta

Predictive value of soluble fibrinogen-like protein 2 for survival in traumatic patients with sepsis

日期：2021-10-20 IF：2.695
Bioengineered

Knockdown of lncRNA PCAT6 suppresses the growth of non-small cell lung cancer cells by inhibiting macrophages M2 polarization via miR-326/KLF1 axis

日期：2022-05-24 IF：3.269
Drug Development Research

Knockdown of IGF2BP3 inhibits the tumorigenesis of gallbladder cancer and modifies tumor microenvironment

日期：2022-10-02 IF：5.004
bioRxiv

Exosomes derived from BMSCs with miR-124-3p inhibitor protects against LPS-induced endometritis through regulation of DUSP6, p-p65 and p-ERK

日期：2023-02-02 IF：-

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
500.00	2.079	1.998
250.00	1.556	1.475
125.00	1.087	1.006
62.50	0.735	0.654
31.25	0.559	0.478
15.63	0.361	0.280
7.82	0.187	0.106
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL10 and the recovery rates were calculated by comparing the measured value to the expected amount of IL10 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	83-98%	91%
Heparin plasma(n=5)	91-99%	95%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.