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大鼠白介素1α(IL1a)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1148

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Rat IL1a(Interleukin 1 Alpha) ELISA Kit
Reactivity:	Rat
Alternative Names:	IL1 Alpha; IL1-A; IL-1α;IL-1a；?IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
Assay Type:	Sandwich
Sensitivity:	6.1 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Apoptosis;
Uniprot ID:	P16598
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1a in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Square

Lycopene alleviates inflammatory explosion and oxidative stress in lipopolysaccharide-induced reprodu ctive system injury of male rats

日期：2022-04-28 IF：-
Research Square

Lycopene alleviates inflammatory explosion and oxidative stress in lipopolysaccharide-induced reprodu ctive system injury of male rats

日期：2022-04-28 IF：-
Journal of Inflammation Research

Oral Lycopene Administration Attenuates Inflammation and Oxidative Stress by Regulating Plasma Lipids in Rats with Lipopolysaccharide-Induced Epididymitis

日期：2022-11-29 IF：4.631
International Journal of Traditional and Complementary Medicine Research

Treatment of Burn Wounds with a Chitosan-Based Hydrogel Dressing Containing Artemisia absinthium L.: A Comprehensive In Vivo Study

日期：2024-09-14 IF：1
Journal of Inflammation Research

Is Lipopolysaccharide-Induced Lipid Metabolism Disorder in Testis of Rats a Consequence of Plasma Lipid Changes?

日期：2024-02-07 IF：4.2
International Journal of Traditional and Complementary Medicine Research

文獻(xiàn)標(biāo)題 Treatment of Burn Wounds with a Chitosan-Based Hydrogel Dressing Containing Artemisia absinthium L.: A Comprehensive In Vivo Study

日期：2024-04-29 IF：0
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

Production and Characterization of Electrospun Chitosan, Nanochitosan and Hyaluronic Acid Membranes for Skin Wound Healing

日期：2024-09-26 IF：3.2

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.172	2.075
500.00	1.407	1.310
250.00	1.198	1.101
125.00	0.797	0.700
62.50	0.567	0.470
31.25	0.319	0.222
15.63	0.187	0.090
0.00	0.097	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL1a and the recovery rates were calculated by comparing the measured value to the expected amount of IL1a in samples.

Matrix	Recovery range	Average
serum(n=5)	88-103%	95%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	92-106%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.