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人轉(zhuǎn)化生長因子β1(TGFb1)酶聯(lián)免疫吸附檢測試劑盒

ELK1185

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
Reactivity:	Human
Alternative Names:	TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
Assay Type:	Sandwich
Sensitivity:	5.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P01137
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

WOUND REPAIR AND REGENERATION

Foamy macrophages potentially inhibit tuberculous wound healing by inhibiting the TLRs/NF-κB signalling pathway

日期：2022-04-06 IF：3.617
bioRxiv

Exosomes derived from BMSCs with miR-124-3p inhibitor protects against LPS-induced endometritis through regulation of DUSP6, p-p65 and p-ERK

日期：2023-02-02 IF：
Biomedicines

Comparison between Platelet Lysate, Platelet Lysate Serum, and Fetal Bovine Serum as Supplements for Cell Culture, Expansion, and Cryopreservation

日期：2024-01-02 IF：4.7
Unveiling the protective effects of BMSCs/anti-miR-124-3p exosomes on LPS-induced endometrial injury

Functional & Integrative Genomics

日期：2024-02-16 IF：6.5
Journal of Orthopaedic Surgery and Research

The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis

日期：2024-09-10 IF：2.8
Functional & Integrative Genomics

Astragaloside IV suppresses the migration and EMT progression of cervical cancer cells by inhibiting macrophage M2 polarization through TGFβ/Smad2/3 signaling

日期：2023-04-21 IF：3.67
European Journal of Histochemistry

Tumor cells-derived exosomal PD-L1 promotes the growth and invasion of lung cancer cells in vitro via mediating macrophages M2 polarization

日期：2023-08-01 IF：2
Functional & Integrative Genomics

Unveiling the protective effects of BMSCs/anti-miR-124-3p exosomes on LPS-induced endometrial injury

日期：2024-02-16 IF：2.9
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The Effect of a Rotating Magnetic Field on the Regenerative Potential of Platelets

日期：2024-03-25 IF：4.9

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.950	1.861
500.00	1.631	1.542
250.00	1.139	1.050
125.00	0.815	0.726
62.50	0.519	0.430
31.25	0.372	0.283
15.63	0.226	0.137
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TGFb1 and the recovery rates were calculated by comparing the measured value to the expected amount of TGFb1 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-93%	87%
EDTA plasma(n=5)	82-94%	88%
Heparin plasma(n=5)	85-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFb1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.