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人血管內(nèi)皮生長因子D(VEGFD)酶聯(lián)免疫吸附檢測試劑盒

ELK1195

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Human VEGFD(Vascular Endothelial Growth Factor D) ELISA Kit
Reactivity:	Human
Alternative Names:	FIGF; VEGF-D; C-Fos Induced Growth Factor
Assay Type:	Sandwich
Sensitivity:	5.7 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Bone metabolism;
Uniprot ID:	O43915
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VEGFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFD in the samples is then determined by comparing the OD of the samples to the standard curve.

frontiers in oncology

Curcumin in Combination With Omacetaxine Suppress Lymphoma Cell Growth, Migration, Invasion, and Angiogenesis via Inhibition of VEGF/Akt Signaling Pathway

日期：2021-08-11 IF：4.451
Frontiers in Pharmacology

Benserazide, a cystathionine beta-synthase (CBS) inhibitor, potentially enhances the anticancer effects of paclitaxel via inhibiting the S-sulfhydration of SIRT1 and the HIF1-α/VEGF pathway

日期：2024-05-17 IF：5.6

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.045	1.950
500.00	1.577	1.482
250.00	1.186	1.091
125.00	0.726	0.631
62.50	0.427	0.332
31.25	0.302	0.207
15.63	0.196	0.101
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VEGFD and the recovery rates were calculated by comparing the measured value to the expected amount of VEGFD in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	92-105%	98%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.