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人氨基端前腦鈉素(NT-ProBNP)酶聯(lián)免疫吸附檢測試劑盒

ELK1212

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity:	Human
Alternative Names:	NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type:	Sandwich
Sensitivity:	15 pg/mL
Standard:	2500 pg/mL
Detection Range:	39.07-2500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Diabetes & Vascular Disease Research

Dapagliflozin attenuates cardiac remodeling and dysfunction in rats with β-adrenergic receptor overactivation through restoring calcium handling and suppressing cardiomyocyte apoptosis

日期：2023-07-01 IF：2.4
Cureus Journal of Medical Science

Assessment and Comparison of N-TerminalProbrain Natriuretic Peptide (NT-proBNP) in Saliva and Serum of Healthy Subjects, Periodontitis Patients, and Periodontitis Patients With Myocardial Infarction

日期：2024-08-21 IF：1
Cureus Journal of Medical Science

Assessment and Comparison of N-TerminalProbrain Natriuretic Peptide (NT-proBNP) in Saliva and Serum of Healthy Subjects, Periodontitis Patients, and Periodontitis Patients With Myocardial Infarction

日期：2024-08-21 IF：1

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2500.00	2.037	1.951
1250.00	1.572	1.486
625.00	1.216	1.130
312.50	0.921	0.835
156.25	0.563	0.477
78.13	0.403	0.317
39.07	0.256	0.170
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.

Matrix	Recovery range	Average
serum(n=5)	90-103%	96%
EDTA plasma(n=5)	81-96%	88%
Heparin plasma(n=5)	78-94%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.