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小鼠促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫吸附檢測試劑盒

ELK1223

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	Mouse ACTH(Adrenocorticotropic Hormone) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Corticotropin; Adrenocorticotropin
Assay Type:	Sandwich
Sensitivity:	6.94 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Hormone metabolism;Urology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Ethnopharmacology

The chemometrics analysis and integrated pharmacology approach to decipher the effect and mechanism between raw and processed cistanche tubulosa

日期：2024-06-28 IF：4.8

標準曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.268	2.167
1000.00	1.527	1.426
500.00	1.128	1.027
250.00	0.897	0.796
125.00	0.572	0.471
62.50	0.387	0.286
31.25	0.217	0.116
0.00	0.101	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ACTH and the recovery rates were calculated by comparing the measured value to the expected amount of ACTH in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	86-96%	91%
Heparin plasma(n=5)	89-107%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.