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大鼠生長調(diào)節(jié)致癌基因a(GROa/CXCL1)酶聯(lián)免疫吸附檢測試劑盒

ELK1237

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview

Product name:	Rat GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Reactivity:	Rat
Alternative Names:	CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein; Neutrophil Activating Protein 3
Assay Type:	Sandwich
Sensitivity:	6.7 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P14095
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.038	1.949
500.00	1.609	1.520
250.00	0.974	0.885
125.00	0.659	0.570
62.50	0.447	0.358
31.25	0.352	0.263
15.63	0.203	0.114
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GROa/CXCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of GROa/CXCL1 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	90-103%	96%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GROa/CXCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.