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人纖溶酶原激活物抑制因子1(PAI1)酶聯(lián)免疫吸附檢測試劑盒

ELK1250

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human PAI1(Plasminogen Activator Inhibitor 1) ELISA Kit
Reactivity:	Human
Alternative Names:	SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor
Assay Type:	Sandwich
Sensitivity:	5.1 pg/mL
Standard:	800 pg/mL
Detection Range:	12.5-800 pg/mL
Sample Type:	plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Hematology;
Uniprot ID:	P05121
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAI1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Scientific Reports

Alcohol use disorder disrupts BDNF maturation via the PAI-1 pathway which could be reversible with abstinence

日期：2024-09-29 IF：3.8

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
800.00	2.054	1.971
400.00	1.782	1.699
200.00	1.135	1.052
100.00	0.892	0.809
50.00	0.562	0.479
25.00	0.321	0.238
12.50	0.227	0.144
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PAI1 and the recovery rates were calculated by comparing the measured value to the expected amount of PAI1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	83-97%	90%
Heparin plasma(n=5)	78-92%	85%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAI1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.