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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
50.00 | 2.027 | 1.940 |
25.00 | 1.487 | 1.400 |
12.50 | 1.043 | 0.956 |
6.25 | 0.828 | 0.741 |
3.13 | 0.508 | 0.421 |
1.57 | 0.340 | 0.253 |
0.79 | 0.190 | 0.103 |
0.00 | 0.087 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-99% | 93% |
EDTA plasma(n=5) | 78-92% | 85% |
Heparin plasma(n=5) | 85-99% | 92% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 97-105% | 86-98% | 84-93% | 89-102% |
EDTA plasma(n=5) | 85-94% | 87-105% | 92-101% | 79-90% |
Heparin plasma(n=5) | 85-102% | 82-96% | 87-98% | 78-92% |
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