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犬單核細(xì)胞趨化蛋白1(MCP1)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1334

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview

Product name:	Dog MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
Reactivity:	Dog
Alternative Names:	CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE
Assay Type:	Sandwich
Sensitivity:	6.6 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P52203
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog MCP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog MCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog MCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.968	1.884
500.00	1.636	1.552
250.00	1.183	1.099
125.00	0.827	0.743
62.50	0.505	0.421
31.25	0.324	0.240
15.63	0.211	0.127
0.00	0.084	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MCP1 and the recovery rates were calculated by comparing the measured value to the expected amount of MCP1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	80-95%	87%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MCP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.