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人白介素37(IL37)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1343

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview

Product name:	Human IL-37(Interleukin 37) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-1Z;IL1 Zeta; IL1F7; IL37; FIL1; FIL1(ZETA); FIL1Z; IL-1F7; IL-1H4; IL-1RP1; IL1H4; IL1RP1; Interleukin 37; Interleukin-1-Related Protein; Interleukin 1 Family; Member 7
Assay Type:	Sandwich
Sensitivity:	3.3 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;
Uniprot ID:	Q9NZH6
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	2.172	2.074
250.00	1.588	1.490
125.00	1.106	1.008
62.50	0.835	0.737
31.25	0.539	0.441
15.63	0.384	0.286
7.82	0.247	0.149
0.00	0.098	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-37 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	81-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.