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小鼠成纖維細(xì)胞生長因子21(FGF21)酶聯(lián)免疫吸附檢測試劑盒

ELK1384

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse FGF21(Fibroblast Growth Factor 21) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Fgf21; FGF-21
Assay Type:	Sandwich
Sensitivity:	5.2 pg/mL
Standard:	200 pg/mL
Detection Range:	3.13-200 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;
Uniprot ID:	Q9JJN1
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF21. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FGF21. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FGF21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FGF21 in the samples is then determined by comparing the OD of the samples to the standard curve.

CRITICAL REVIEWS IN IMMUNOLOGY

Increased PKN2 and M2-Polarized Macrophages Promote HCT116 Cell Invasion

日期：2024-01-12 IF：1.3

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
200.00	2.146	2.060
100.00	1.636	1.550
50.00	1.106	1.020
25.00	0.866	0.780
12.50	0.533	0.447
6.25	0.325	0.239
3.13	0.196	0.110
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FGF21 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF21 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-96%	89%
EDTA plasma(n=5)	92-107%	99%
Heparin plasma(n=5)	92-105%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.