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人免疫球蛋白G(IgG)酶聯(lián)免疫吸附檢測試劑盒

ELK1390

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human IgG(Immunoglobulin G) ELISA Kit
Reactivity:	Human
Alternative Names:	Immunoglobulin G
Assay Type:	Sandwich
Sensitivity:	1.54 μg/mL
Standard:	200 μg/mL
Detection Range:	3.13-200 μg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;Immune molecule;Hematology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

LAB ON A CHIP

Bioengineering of a human physiologically relevant microfluidic blood–cerebrospinal fluid barrier model

日期：2023-05-26 IF：6.1

標(biāo)準(zhǔn)曲線

Concentration (μg/mL)	OD	Corrected OD
200.00	1.951	1.871
100.00	1.557	1.477
50.00	1.165	1.085
25.00	0.861	0.781
12.50	0.473	0.393
6.25	0.279	0.199
3.13	0.168	0.088
0.00	0.080	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IgG and the recovery rates were calculated by comparing the measured value to the expected amount of IgG in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	92-106%	100%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.