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小鼠甲狀腺球蛋白(TG)酶聯(lián)免疫吸附檢測試劑盒

ELK1446

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse TG(Thyroglobulin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	AITD3; TGN
Assay Type:	Sandwich
Sensitivity:	13.3 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;
Uniprot ID:	O08710
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TG in the samples is then determined by comparing the OD of the samples to the standard curve.

Food & Function

Egg white-derived peptides reduced blood glucose in high-fat-diet and low-dose streptozotocin-induced type 2 diabetic mice via regulating the hepatic gluconeogenic signaling and metabolic profile

日期：2024-06-07 IF：6.1

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.045	1.964
1000.00	1.502	1.421
500.00	1.115	1.034
250.00	0.757	0.676
125.00	0.583	0.502
62.50	0.426	0.345
31.25	0.226	0.145
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TG and the recovery rates were calculated by comparing the measured value to the expected amount of TG in samples.

Matrix	Recovery range	Average
serum(n=5)	88-106%	97%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	82-97%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.