免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

產(chǎn)品中心

大鼠腫瘤蛋白p53(TP53)酶聯(lián)免疫吸附檢測試劑盒
ELK1509
規(guī)格: 價格:
48T ¥1960.00
96T ¥2800.00

Overview

Product name: Rat TP53(Tumor Protein p53) ELISA Kit
Reactivity: Rat
Alternative Names: p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Assay Type: Sandwich
Sensitivity: 31 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Tumor immunity;
Uniprot ID: P10361
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TP53. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TP53 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL) OD Corrected OD
5000.00 2.169 2.075
2500.00 1.577 1.483
1250.00 1.106 1.012
625.00 0.835 0.741
312.50 0.538 0.444
156.25 0.342 0.248
78.13 0.241 0.147
0.00 0.094 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TP53 and the recovery rates were calculated by comparing the measured value to the expected amount of TP53 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 83-99% 95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TP53 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 93-106% 85-95% 92-104%
EDTA plasma(n=5) 82-96% 95-102% 87-98% 81-97%
Heparin plasma(n=5) 88-97% 93-103% 94-106% 91-101%
關閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術支持

關注微信公眾號

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關閉
安义县| 澄迈县| 疏勒县| 米林县| 凤凰县| 九龙县| 乡城县| 临潭县| 镇巴县| 阿坝县| 图们市| 桃江县| 南开区| 郁南县| 即墨市| 旬邑县| 岢岚县| 陆河县| 宁明县| 册亨县| 泸州市| 绿春县| 普格县| 东至县| 财经| 贺州市| 安庆市| 师宗县| 德州市| 中牟县| 台东县| 普兰店市| 左权县| 长海县| 文登市| 西吉县| 崇左市| 图们市| 应用必备| 德化县| 天祝|