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人胱天蛋白酶9(CASP9)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1530

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human CASP9(Caspase 9) ELISA Kit
Reactivity:	Human
Alternative Names:	APAF3; ICE-LAP6; MCH6; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 9; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6
Assay Type:	Sandwich
Sensitivity:	0.117 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Infection immunity;
Uniprot ID:	P55211
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CASP9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CASP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CASP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CASP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (ng/mL)	OD	Corrected OD
20.00	2.285	2.202
10.00	1.783	1.700
5.00	1.251	1.168
2.50	0.806	0.723
1.25	0.539	0.456
0.63	0.363	0.280
0.32	0.258	0.175
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CASP9 and the recovery rates were calculated by comparing the measured value to the expected amount of CASP9 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	78-96%	87%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CASP9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.