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大鼠骨橋素(OPN)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1540

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Rat OPN(Osteopontin) ELISA Kit
Reactivity:	Rat
Alternative Names:	SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
Assay Type:	Sandwich
Sensitivity:	0.128 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Bone metabolism;
Uniprot ID:	P08721
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OPN in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (ng/mL)	OD	Corrected OD
20.00	2.132	2.051
10.00	1.587	1.506
5.00	1.168	1.087
2.50	0.864	0.783
1.25	0.567	0.486
0.63	0.340	0.259
0.32	0.194	0.113
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OPN and the recovery rates were calculated by comparing the measured value to the expected amount of OPN in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	80-97%	88%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.