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大鼠骨鈣素(OC)酶聯(lián)免疫吸附檢測試劑盒

ELK2391

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Rat OC(Osteocalcin) ELISA Kit
Reactivity:	Rat
Alternative Names:	BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Assay Type:	Sandwich
Sensitivity:	0.28 ng/mL
Standard:	50 ng/mL
Detection Range:	0.78-50 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Uniprot ID:	P04640
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OC in the samples is then determined by comparing the OD of the samples to the standard curve.

Fermentation

Development and Validation of Flaxseed Lignan-Enriched Set-Type Fermented Milk to Manage Postmenopausal Osteoporosis

日期：2024-01-04 IF：3.7
Nigerian Journal of Clinical Practice

Effect of the Combination of Exercise and Metformin on Osteocalcin, Insülin, Interleukin-6, Glucose Levels, and Body Weights in Rats

日期：2024-06-15 IF：0.7

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
50.00	2.126	2.044
25.00	1.673	1.591
12.50	0.997	0.915
6.25	0.682	0.600
3.13	0.492	0.410
1.57	0.332	0.250
0.79	0.197	0.115
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OC and the recovery rates were calculated by comparing the measured value to the expected amount of OC in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.