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大鼠促腎上皮質(zhì)激素釋放激素(CRH)酶聯(lián)免疫吸附檢測試劑盒

ELK2606

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Rat CRH(Corticotropin Releasing Hormone) ELISA Kit
Reactivity:	Rat
Alternative Names:	CRF; Corticotropin Releasing Factor; Corticoliberin
Assay Type:	Competitive Inhibition
Sensitivity:	4.95 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;
Uniprot ID:	P01143
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Experimental Neurology

CRHR1 antagonist alleviated depression-like behavior by downregulating p62 in a rat model of post-stroke depression

日期：2024-07-25 IF：4.6

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	0.235
500.00	0.427
250.00	0.711
125.00	0.959
62.50	1.275
31.25	1.565
15.63	1.889
0.00	2.197

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CRH and the recovery rates were calculated by comparing the measured value to the expected amount of CRH in samples.

Matrix	Recovery range	Average
serum(n=5)	88-103%	95%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.