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大鼠層粘連蛋白(LN)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK3050

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Rat LN(Laminin) ELISA Kit
Reactivity:	Rat
Alternative Names:	LAM
Assay Type:	Sandwich
Sensitivity:	13.8 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Signal Transduction
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LN in the samples is then determined by comparing the OD of the samples to the standard curve.

Pharmaceuticals

Phenylethanol Glycoside from Cistanche tubulosa Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis

日期：2024-08-30 IF：4.3

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.056	1.970
1000.00	1.631	1.545
500.00	1.106	1.020
250.00	0.842	0.756
125.00	0.529	0.443
62.50	0.332	0.246
31.25	0.219	0.133
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LN and the recovery rates were calculated by comparing the measured value to the expected amount of LN in samples.

Matrix	Recovery range	Average
serum(n=5)	80-96%	88%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	83-99%	95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.