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小鼠鈣網(wǎng)蛋白(CRT)酶聯(lián)免疫吸附檢測試劑盒

ELK3182

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Mouse CRT(Calreticulin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CALR; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60
Assay Type:	Sandwich
Sensitivity:	0.067 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Metabolic pathway;Bone metabolism;
Uniprot ID:	P14211
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRT in the samples is then determined by comparing the OD of the samples to the standard curve.

Materials Today Nano

Metabolic Regulating Enhanced Immunological Activating Nanocomposites for Tumor Microenvironment Normalization and Immunotherapy

日期：2024-08-10 IF：8.2

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.166	2.089
5.00	1.621	1.544
2.50	0.997	0.920
1.25	0.777	0.700
0.63	0.532	0.455
0.32	0.351	0.274
0.16	0.206	0.129
0.00	0.077	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CRT and the recovery rates were calculated by comparing the measured value to the expected amount of CRT in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	89-103%	96%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.