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小鼠S100鈣結合蛋白A8(S100A8)酶聯(lián)免疫吸附檢測試劑盒

ELK3284

規格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻

Product name:	Mouse S100A8(S100 Calcium Binding Protein A8) ELISA Kit
Reactivity:	Mouse
Alternative Names:	MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8
Assay Type:	Sandwich
Sensitivity:	2.9 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Infection immunity;
Uniprot ID:	P27005
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100A8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100A8 in the samples is then determined by comparing the OD of the samples to the standard curve.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

High-dose vitamin C attenuates radiation-induced pulmonary fibrosis by targeting S100A8 and S100A9

日期：2024-07-10 IF：4.2

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
500.00	1.932	1.851
250.00	1.602	1.521
125.00	1.185	1.104
62.50	0.896	0.815
31.25	0.539	0.458
15.63	0.239	0.158
7.82	0.208	0.127
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant S100A8 and the recovery rates were calculated by comparing the measured value to the expected amount of S100A8 in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	80-95%	87%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100A8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.