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小鼠促卵泡素(FSH)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK4808

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Mouse FSH(Follicle Stimulating Hormone) ELISA Kit
Reactivity:	Mouse
Alternative Names:	FSH-B; FSH-beta
Assay Type:	Sandwich
Sensitivity:	0.67 mIU/mL
Standard:	100 mIU/mL
Detection Range:	1.57-100 mIU/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FSH in the samples is then determined by comparing the OD of the samples to the standard curve.

Metabolism Open

Turmeric extract alleviates endocrine-metabolic disturbances in letrozole-induced PCOS by increasing adiponectin circulation: A comparison with Metformin

日期：2021-11-23 IF：-
Obesity Medicine

Metformin treatment ameliorates endocrine-metabolic disturbances in letrozole-induced PCOS mice model by modulating adiponectin status

日期：2022-03-04 IF：1.5
Obesity Medicine

Metformin treatment ameliorates endocrine-metabolic disturbances in letrozole-induced PCOS mice model by modulating adiponectin status

日期：2022-03-04 IF：1.5
Comparative Clinical Pathology

Ameliorative effects of quercetin on endocrine and metabolic abnormalities associated with experimentally induced polycystic ovary syndrome in mice

日期：2023-02-08 IF：-
BMC Endocrine Disorders

Diacerein mitigates endocrine and cardio-metabolic disruptions in experimental PCOS mice model by modulating AdipoR1/ PON 1

日期：2024-07-14 IF：2.8

標(biāo)準(zhǔn)曲線

Concentration (mIU/mL)	OD	Corrected OD
100.00	2.013	1.932
50.00	1.700	1.619
25.00	1.240	1.159
12.50	0.796	0.715
6.25	0.517	0.436
3.13	0.321	0.240
1.57	0.175	0.094
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FSH and the recovery rates were calculated by comparing the measured value to the expected amount of FSH in samples.

Matrix	Recovery range	Average
serum(n=5)	95-108%	101%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	80-93%	89%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.