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大鼠核因子κB亞基p105(NFKB-p105)酶聯(lián)免疫吸附檢測試劑盒

ELK5691

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Rat NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
Reactivity:	Rat
Alternative Names:	NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Type:	Sandwich
Sensitivity:	0.114 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	Q63369
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

INFLAMMATION

GFI-1 Protects Against Lipopolysaccharide-Induced Inflammatory Responses and Apoptosis by Inhibition of the NF-κB/TNF-α Pathway in H9c2 Cells

日期：2021-10-20 IF：3.211
Experimental and Therapeutic Medicine

The effects of compound centella formula on OxInflammation and silent information regulator 1 in a high?fat diet/streptozotocin?induced diabetic kidney disease rat model

日期：2021-07-07 IF：2.447
Cellular and Molecular Biology

Impacts of trelagliptin and remogliflozin alone and in combination with Alpha Lipoic Acid on cardiac function in streptozotocin-induced diabetes mellitus in rats

日期：2023-10-08 IF：1.6
BMC Surgery

Management of metabolic and reproductive disturbances in letrozole induced polycystic ovarian syndrome by Asparagus racemosus roots

日期：2024-07-11 IF：1.9
Journal of Ovarian Research

Mitigation of letrozole induced polycystic ovarian syndrome associated inflammatory response and endocrinal dysfunction by Vitex negundo seeds

日期：2024-04-08 IF：3.8
Journal of medicine and life

Comparative treatment of Sulfasalazine+Ezetimibe combination and Sulfasalazine in a rat model with induced colitis

日期：2023-08-01 IF：1
Medical Journal of Babylon

The Effects of Sulfasalazine and Ezetimibe on Proinflammatory Cytokines in Male Rat with Induced Colitis: A Comparative Study

日期：2024-09-30 IF：0

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.379	2.296
10.00	1.621	1.538
5.00	1.115	1.032
2.50	0.735	0.652
1.25	0.555	0.472
0.63	0.298	0.215
0.32	0.223	0.140
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NF-κBp105 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κBp105 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	81-93%	87%
Heparin plasma(n=5)	82-94%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κBp105 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.