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小鼠殺菌肽LL-37(LL-37)酶聯(lián)免疫吸附檢測試劑盒

ELK7205

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

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Overview 文獻(xiàn)

Product name:	Mouse LL-37(Antibacterial Protein LL-37) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
Assay Type:	Sandwich
Sensitivity:	0.28 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

Tuberculosis

Macrophage-targeted versus free calcitriol as host-directed adjunct therapy against Mycobacterium tuberculosis infection in mice is bacteriostatic and mitigates tissue pathology

日期：2024-07-17 IF：2.8

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
50.00	2.126	2.038
25.00	1.674	1.586
12.50	1.296	1.208
6.25	0.869	0.781
3.13	0.625	0.537
1.57	0.301	0.213
0.79	0.234	0.146
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-99%	95%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	85-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.