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小鼠胃動素(MTL)酶聯(lián)免疫吸附檢測試劑盒

ELK7634

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Mouse MTL(Motilin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	MLN;? MAP; Promotilin; Motilin-associated peptide
Assay Type:	Competitive Inhibition
Sensitivity:	9.52 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Gastroenterology;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MTL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MTL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MTL in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Ethnopharmacology

The chemometrics analysis and integrated pharmacology approach to decipher the effect and mechanism between raw and processed cistanche tubulosa

日期：2024-06-28 IF：4.8

標準曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	0.191
1000.00	0.310
500.00	0.451
250.00	0.598
125.00	0.763
62.50	1.439
31.25	1.724
0.00	2.475

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MTL and the recovery rates were calculated by comparing the measured value to the expected amount of MTL in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	87-95%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MTL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.