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5-羥色胺(5-HT)酶聯(lián)免疫吸附檢測試劑盒

ELK7830

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻

Product name:	5-HT(5-Hydroxytryptamine) ELISA Kit
Reactivity:	General
Alternative Names:	5HT; Serotonin; N-Methyl-Gamma
Assay Type:	Competitive Inhibition
Sensitivity:	3.97 ng/mL
Standard:	900 ng/mL
Detection Range:	14.07-900 ng/mL
Sample Type:	serum, plasma, tissue homogenates
Assay Length:	2h
Research Area:	Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Ciências Agrárias

Efeitos de bactérias probióticas sobre o bem-estar de matrizes suínas e de leit?es lactentes

日期：2024-08-02 IF：0.4
Small Ruminant Research

Behavioural and neuroendocrine responses in alpacas (Vicugna pacos) to the shearing procedure

日期：2024-10-22 IF：1.6

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
900.00	0.183
450.00	0.385
225.00	0.575
112.50	0.823
56.25	1.157
28.13	1.592
14.07	1.624
0.00	2.256

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HT in samples.

Matrix	Recovery range	Average
serum(n=5)	88-103%	95%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	80-95%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.