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環(huán)磷酸腺苷(cAMP)酶聯(lián)免疫吸附檢測試劑盒

ELK8116

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	cAMP(Cyclic Adenosine Monophosphate) ELISA Kit
Reactivity:	General
Alternative Names:	c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate
Assay Type:	Competitive Inhibition
Sensitivity:	92.5 pg/mL
Standard:	20000 pg/mL
Detection Range:	312.5-20000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Signal transduction;Metabolic pathway;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cAMP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.

Experimental and Therapeutic Medicine

cAMP regulates 11β?hydroxysteroid dehydrogenase?2 and Sp1 expression in MLO?Y4/MC3T3?E1 cells

日期：2021-10-21 IF：1.73
Nutrients

Silver Carp (Hypophthalmichthys molitrix) Scale Collagen Peptides-1 (SCPs1) Inhibit Melanogenesis through Downregulation of the cAMP-CREB Signaling Pathway

日期：2023-05-19 IF：6.706
Journal of Ethnopharmacology

The chemometrics analysis and integrated pharmacology approach to decipher the effect and mechanism between raw and processed cistanche tubulosa

日期：2024-06-28 IF：4.8

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
20000.00	0.237
10000.00	0.411
5000.00	0.715
2500.00	0.913
1250.00	1.225
625.00	1.579
312.50	1.813
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant cAMP and the recovery rates were calculated by comparing the measured value to the expected amount of cAMP in samples.

Matrix	Recovery range	Average
serum(n=5)	93-105%	99%
EDTA plasma(n=5)	84-93%	89%
Heparin plasma(n=5)	81-103%	94%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cAMP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.