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大鼠殺菌肽LL-37(LL-37)酶聯(lián)免疫吸附檢測試劑盒

ELK8207

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Rat LL-37(Antibacterial Protein LL-37) ELISA Kit
Reactivity:	Rat
Alternative Names:	CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
Assay Type:	Sandwich
Sensitivity:	57 pg/mL
Standard:	8000 pg/mL
Detection Range:	125-8000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

researchsquare

Adenosine A2a receptor mediate anti-inammation and collagen protection via cAMP/CREB/NFκB pathway in nucleus pulposus cells

日期：2022-12-13 IF：-
European Journal of Medical Research

A2AR regulate inflammation through PKA/NF-κB signaling pathways in intervertebral disc degeneration

日期：2024-08-27 IF：2.8

標準曲線

Concentration (pg/mL)	OD	Corrected OD
8000.00	2.052	1.965
4000.00	1.717	1.630
2000.00	1.282	1.195
1000.00	0.797	0.710
500.00	0.535	0.448
250.00	0.324	0.237
125.00	0.175	0.088
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	88-102%	95%
Heparin plasma(n=5)	83-98%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.