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大麻素(AEA)酶聯(lián)免疫吸附檢測試劑盒

ELK8269

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	AEA(Anandamide) ELISA Kit
Reactivity:	General
Alternative Names:	N-Arachidonoylethanolamine
Assay Type:	Competitive Inhibition
Sensitivity:	0.94 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Neuro science; Immunity
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AEA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AEA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AEA in the samples is then determined by comparing the OD of the samples to the standard curve.

Clinical Chemistry and Laboratory Medicine

Cardiovascular diseases and cardiac biomarkers

日期：2023-05-22 IF：6.8
Applied Sciences

Endogenous Cannabinoids in Crohn’s Disease

日期：2024-07-30 IF：

標準曲線

Concentration (ng/mL)	OD	Corrected OD
200.00	0.199
100.00	0.385
50.00	0.621
25.00	0.998
12.50	1.253
6.25	1.522
3.13	1.786
0.00	2.375

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant AEA and the recovery rates were calculated by comparing the measured value to the expected amount of AEA in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	92-107%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AEA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.