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大鼠糖胺聚糖(GAGs)酶聯(lián)免疫吸附檢測試劑盒

ELK8486

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Rat GAGs(Glycosaminoglycan) ELISA Kit
Reactivity:	Rat
Alternative Names:	Mucopolysaccharide; GAGs;
Assay Type:	Competitive Inhibition
Sensitivity:	0.51 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Tumor immunity;Hepatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GAGs protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GAGs. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GAGs in the samples is then determined by comparing the OD of the samples to the standard curve.

Langmuir

Plunge-Freezing Cryopreservation of Tendons

日期：2024-06-25 IF：3.7

標準曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	0.199
50.00	0.389
25.00	0.593
12.50	0.861
6.25	1.157
3.13	1.537
1.57	1.789
0.00	2.256

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GAGs and the recovery rates were calculated by comparing the measured value to the expected amount of GAGs in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	85-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAGs and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.