產(chǎn)品中心

人細(xì)胞角蛋白18(CK-18)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK8576

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human CK-18(Cytokeratin 18) ELISA Kit
Reactivity:	Human
Alternative Names:	Cell proliferation inducing gene 46 protein ; Cell proliferation inducing protein 46 ; Cell proliferation-inducing gene 46 protein ; CK 18 ; CK-18 ; CK18 ; CYK 18 ; CYK18 ; Cytokeratin 18 ; Cytokeratin endo B ; Cytokeratin-18 ; K 18 ; K18 ; K1C18_HUMAN ; KA18 ; Keratin 18 ; Keratin 18, type I ; Keratin D ; keratin, type I cytoskeletal 18 ; Keratin-18 ; Krt18
Assay Type:	Sandwich
Sensitivity:	0.058 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Cancer of the liver; Viral hepatitis
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK-18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK-18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK-18 in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Clinical Medicine

Impact of Comorbid Polycystic Ovary Syndrome on Clinical and Laboratory Parameters in Female Adolescents with Metabolic Dysfunction-Associated Steatotic Liver Disease: A Cross-Sectional Study

日期：2024-10-02 IF：3

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	1.933	1.840
5.00	1.697	1.604
2.50	1.037	0.944
1.25	0.838	0.745
0.63	0.509	0.416
0.32	0.318	0.225
0.16	0.187	0.094
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CK-18 and the recovery rates were calculated by comparing the measured value to the expected amount of CK-18 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	78-96%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.