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大鼠食欲素(OX)酶聯(lián)免疫吸附檢測試劑盒

ELK8935

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Rat OX(Orexin) ELISA Kit
Reactivity:	Rat
Alternative Names:	HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor
Assay Type:	Competitive Inhibition
Sensitivity:	4.53 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	2h
Research Area:	Neuro science;
Uniprot ID:	O55232
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat OX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OX in the samples is then determined by comparing the OD of the samples to the standard curve.

Molecular Biology Reports

Role of Thymoquinone on sleep restriction and its mitigating effect on leptin-mediated signaling pathway in rat brain

日期：2024-06-14 IF：2.6

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	0.227
500.00	0.376
250.00	0.612
125.00	0.833
62.50	1.157
31.25	1.585
15.63	1.793
0.00	2.028

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OX and the recovery rates were calculated by comparing the measured value to the expected amount of OX in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	83-98%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.